Platelet Counts Analysis of Platelet-Poor Plasma (PPP) Produced by Several Centrifugation Techniques

Abstract

Platelet-poor plasma (PPP) can be obtained by various centrifugation techniques. This study aims to analyze

platelet count in PPP produced by three different centrifugation techniques. Samples came from a healthy

adult who underwent medical check-up and had been given informed consent. A total of 2.7 mL of blood

samples were collected using three citrated tubes. Samples were centrifuged by three different techniques

to obtain PPP: 1,500 g for 15 minutes twice, 3,000 g for 15 minutes, and 3,260 g for 10 minutes. The

platelet count of each PPP was examined using a hematology analyzer. All three centrifugation techniques

produced <10,000/µL platelets in all PPP from 31 samples. The twice centrifugation of 1,500 g for 15

minutes produced a median number of platelets which was 1×103 /µL (0-3). The 3,000 g centrifugation for 15

minutes produced a median number of platelets which was 1×103 /µL (0-5). The 3,260 g centrifugation for 10

minutes produced a median number of platelets which was 2×103 /µL (0-5). A comparison of platelet count

showed a significant difference (p<0.05) among the three centrifugation techniques. The three centrifugation

techniques in this study were able to produce PPP. The centrifugation technique of 1,500 g for 15 minutes

twice produced the lowest number of platelets.