Therapeutic Effect of Statins and LPS Antibody on  Proinflammatory Mediator and Biochemical Markers of Sepsis  Rat Model Induced by E. coli

Authors

Dian Samudra1,2, Sumarno Reto Prawiro3 , Sanarto Santoso3 , Aswoco A. Asmoro4 , Husnul Khotimah5 , Arif N. M. Ansori6 , Emy K. Sabdoningrum6,7

antibody, LPS, E. coli, proinflammatory mediator, biochemistry marker, statins

This study aims to determine the effect of statins and LPS antibodies administration combined with E. coli

intraperitoneal injection toward proinflammatory mediators (TNF-?, hs-CRP, PCT, and MDA) urea, BUN,

and creatinine) and liver function (SGPT, SGOT, and total bilirubin) in sepsis rats. This study used 30 male

white rats (Rattus novergicus strain Wistar) divided into 6 groups induced by E. coli bacteria combined with

statins, LPS antibody (Ab-LPS), and a combination of statins+Ab-LPS. Examination of proinflammatory

mediators (TNF-?, hs-CRP, PCT, and MDA) was carried out by the ELISA method through the examination

of biochemical markers namely renal function (urea, BUN, and creatinine) and liver function (SGPT, SGOT,

and total bilirubin) were analyzed by using the autoanalyzer. The data obtained were statistically analyzed

using the T-test. The result shows that E. coli+statins gave significant changes on the MDA level at 0th hour

administration and TNF-? and hs-CRP at 3rd hour administration. The E. coli+Ab-LPS treatment showed

significant changes at the 0th hour administration for MDA, creatinine, and total bilirubin and at the 3rd

hour administration for MDA. The E. coli+statins+Ab-LPS treatment showed significant changes in MDA,

creatinine, and total bilirubin at 0th hour administration and on creatinine and total bilirubin at 3rd hour

administration.