Investigation the Association of the VEGFR-2 and -2578C\A Polymorphism as a Risk Factor for Incidence of Lung Cancer in Babylon Province

Abstract

Background: VEGF gene polymorphisms can induce either increase or inhibition of VEGF secretion, with

altered promoter activity.

Aim: We investigated the association of the VEGFR-2 and -2578C\A polymorphism in the VEGF gene with

Lung cancer risk in Babylon province. Methodology: VEGFR-2 level was measured by Enzyme Linked

Immunoabsorbant Assay ELISA and Genotyping of the VEGF-gene variation (-2578A\C) was performed

using the amplification refractory mutation system PCR. We investigated the association of VEGF gene

variants with different clinicopathological features of lung cancer patients.

Results: No significant difference was seen of the VEGFR-2 levels in lung cancer patients when compared

with control group (P=092), the allelic frequency of -2578 A\C VEGF gene no difference was seen in patients

lung cancer (p=0.652). Hardy-Weinberg equilibrium showed a significant difference cases group (p=0.016),

also revealed A allele exhibits a pathological behavior and it appears in over-dominant model (p=0.05), this

proves that there is significant association of -2578A\C VEGF gene in lung cancer patients.

Conclusion: Our results showed no significant association of the VEGFR-2 in lung cancer patients

while showed a significant association of the VEGF -2578C\A polymorphism with LC susceptibility in

Babylon province .The VEGF -2578C\A heterozygote significantly increases the risk and can be useful as a

predisposing genetic marker.