Human Age Estimation Through DNA Metilation Analysis Method from Bite Mark Samples in Forensic Identification

Abstract

Background: Age estimation are often calculated using bone and teeth samples, but are limited to human
skeleton findings. The aim of this research is to estimate human age through DNA methylation method
from bite mark samples. Result: There were 40 bite mark samples obtained from healthy volunteers. DNA
samples were isolated using DNAzol reagent and converted using DNA methylation kit. The isolated DNA
were amplified and electrophoreses was conducted using agarose gel. Electrophoresis result was used as
length reference for the sequenced band and analyzed for methylation percentage and correlation with
age estimation. Statistical test showed that there was a significant correlation between DNA methylation
percentage with age estimation both in men (r Pearson 0.767) and women (r Pearson 0.878). Conclusion:
Cpgplot emboss analysis for DNA methylation mean percentage in men tend to increase in accordance to
age categories, whereas for women, the mean DNA methylation percentage in age categories was stable or
constant.