Mitigation of the Biofilm Using Nanoparticles By Enhanced by D-alanine and D-proline

Abstract

The present study was undertaken to investigate the susceptibility patterns as antifungal and growth of certain
D-amino acids inhibitory effects, includes D-alanine(D-ala) and D-proline (D-pro). The obtained results
indicated that D- proline acid is potent mostly as antifungal, among tested D-amino acids, then followed
by D-ala. The study aim was evaluating the of D-amino acids and nanoparticles against Candida albicans
adhered cells and bio-films. Results showed that D-ala MIC value was 25 ?g/ml, while in methods of TCP,
(D-pro) MIC 50 ?g/ml value. Nanoparticles (lithium and silver) and amino acids (D-pro and D-ala) effect
in preventing cells adhesion on polystyrene surface and mature bio-film inhibition was studied. Highest
inhibition was obtained at D-pro concentration 50 ?g /ml, while the lowest at concentration D-ala 25 ?g /ml
against mature bio-film and cell adhesion.