Expression of FOXP1 and p53 in Reactive Lymphoid Lesion and B-cell Non-Hodgkin Lymphoma, Large Cell Type

Abstract

Lymphoproliferative lesions that have morphology between benign and malignant are difficult to diagnose
even with immunohistochemical and clonality testing. The correct diagnosis is necessary for the prompt
treatment. These lesions can also serve as instructive models of lymphomagenesis. FOXP1 plays an important
role in B-cell development, has a potential oncogene in B-cell Non-Hodgkin lymphoma, and p53 protein
has a crucial role in the regulation of cell cycle, DNA repair, apoptosis, and senescence tumor suppression
activity. In this study, we analyze the role of FOXP1 and p53 expression in reactive lymphoid hyperplasia
and B-cell Non-Hodgkin lymphoma, large cell type. 68 paraffin blocks samples from patients diagnosed
as reactive lymphoid hyperplasia and B-cell Non-Hodgkin lymphoma, large cell type was sectioned and
stained with immunohistochemistry for FOXP1 and p53, and the percentage of nuclear cells showing
positive staining were evaluated. Expression of FOXP1 and p53 in B-cell Non-Hodgkin lymphoma, large
cell type is higher than in reactive lymphoid hyperplasia with p=0.001 and cutoff point 45%(CI=95%) for
FOXP1 and p=0.001 and cutoff point 7.5%(CI=95%) for p53. There is a significant correlation between the
expression of FOXP1 and p53 in reactive lymphoid hyperplasia and B-cell Non-Hodgkin lymphoma, large
cell type (p=0.001). Our findings suggest that high expression of FOXP1 and p53 in B-cell Non-Hodgkin
lymphoma may demonstrate the role of FOXP1 and p53 in lymphomagenesis and these markers may help
to distinguish benign and malignant lymphoproliferative lesions.