Comparison of Success Rate of Estrus and Pregnancy between Laser Puncture and Intra Vaginal Progesterone Sponge in Bali Cattle

Abstract

The purpose of this study was to find a method of determining laser models and intra vaginal progesterone
sponge in Bali Cattle. An assessment was carried out at Sapi Loka Grati, Pasuruan, Indonesia. The parameters
observed included the symptoms of estrus, response of estrus, and the level of pregnancy. Twenty Balinese
cows were divided at random in two groups. P1 treated with laser puncture at a certain point and P2 treated
with 1.5 g of progesterone + 10 mg of estradiol benzoate intra vaginal sponge. The diagnosis of subsequent
pregnancies was done by ultrasonographic examination on day 40th after artificial insemination (AI). The
laser used in this study was a soft laser with power supply specifications as follows: 50 Hz, 220 Volts, 50
Watts, ranging from 0.2 Joules to 0.5 Joules. These lasers include semiconductor lasers and laser probes.
Three acupuncture points were used (GV 4, BL-22, and GV-2). Furthermore, the intra vaginal progesterone
sponge used was made from silicone plastic similar to the drugs containing medroxyprogesterone acetate.
Data testing was done by using the T-test. Results demonstrated that the P1 responses of estrus appeared on
day 3rd, while P2 on day 9th. There was no significant difference (p >0.05) in the accumulation of estrus and
pregnancy. In conclusion, estrus synchronization and pregnancy rate could be efficiently achieved in Bali
cattle using either laser puncture or intra vaginal progesterone sponge. However, laser puncture treatment
induced more spread estrus with higher pregnancy rate.