A Reliable Quantification Method for Trimethoprim in Pharmaceutical Samples by HILIC-HPLC

Abstract

Trimethoprim is sometimes used in conjunction with sulfonamides for drug therapies Herein. A practicable
method has been developed to determine trimethoprim in pharmaceutical formulations by hydrophilic
interaction liquid chromatography coupled with an ultraviolet detector. The development and investigation
of a new HLIC assay of trimethoprim. The separation was made in the HALO-HILIC column using a
NaOAc/HAc buffer (40 mM-pH 4.75) with acetonitrile (10:90) (v/v) as mobile phase and was quantified
by UV detection at 280 nm. The limit of quantification was about 0.0242 ppm and the limit of detection
0.008 ppm. The calibration curves were linear in the investigated range (0.01-3 ppm). The recovery of
trimethoprim was 99-102.5%. The method was applied in pharmaceutical formulations.