Phenotypic and molecular characteristics of biofilm and other virulence genes in E. coli and K. pneumoniae isolates from healthy dairy cow, human and environmental sources

Abstract

Escherichia coli and Klebsiella pneumoniae for ones role in opportunistic infections and pathogenic methods
in veterinary and human medicine, including the formation of biofilms. 33 E.coli and 8 K. pneumoniae
isolates of these micro-organisms isolated from cow’s milk, stools and utensils. Identification were done by
biochemical reaction and confirmed by Polymerase Chain (PCR). These isolates exhibit biofilms formation,
as strong, moderate, weak capacities. The expression of the fimA gene. The percentage (84.8%)28/33 of
E.coli was able to produce biofilm while (100%)8/8 in K. pneumoniae The virulence factors of all bacterial
isolates were also studied to determine hylA and bla-CTX-M genes in E.coli and, magA and bla-CTX-M
genes in K. pneumoniae were results presence extended spectrum ?- lactamases (ESBLs). bla-CTX-M gene
in E. coli and K. pneumoniae (78.7% , 87.5%) It also emerged that not all isolates carry other virulence genes
for this study