Hepato-nephroprotective Role of Lepidium sativum against Oxidative Stress Induced by Dexamethasone in Rats

Abstract

The current research was prepared to explore the hepato-nephroprotective effect of Lepidium sativum seed
aquatic extract against oxidative stress stimulated by dexamethasone in rats. Animals were classified as
follows; group one was used as control while groups 2, 3 and 4 were treated orally with Lepidium sativum
seed extract (LSSE; 20 mg/kg, daily), interperitoneally with dexamethasone (DEX; 1 mg/kg BW) and
LSSE plus DEX for 14 days, respectively. Administration of DEX elevated thiobarbituric acid reactive
substances (TBARS), hydrogen peroxide (H2O2), and kidney and liver function biomarkers level, and
lactate dehydrogenase activity. While enzymatic (SOD, CAT, GPx, GR, GST) and non-enzymatic (GSH)
antioxidants, protein content, and alkaline phosphatase activity were significantly decreased. Otherwise, rats
supplemented with LSSE singly declined lipid peroxidation and improved most of the studied parameters.
Moreover, rats pretreated with LSSE then received DEX showed significant alleviation in lipid peroxidation,
antioxidant status and biochemical indices with respect to DEX treated group. Conclusively, LSSE has
beneficial protective effects and has the capability to counteract the harmful influence of DEX. So, Lepidium
sativum might represent a novel approach in the therapy of dexamethasone because of its antioxidant
properties.