Genotype Frequency of Cytochrome P450 2C9 (CYP2C9) Mutations in Iraqi Ethnic Groups

Abstract

In Middle East, There is a little knowledge about the cytochrome P450s genotyping and there has not found
any investigation about the CYP2C9 allelic diversity in Iraqi population nowadays .CYP2C9 genotypes were
established in a total of 400 subjects of healthy Iraqis volunteers from different areas in Iraq (Arab ,Kurd
and Turkmen). After extraction of DNA from blood cells and polymerase chain reaction, SNP genotyping
for CYP2C9 variants (2,3,4 and 5) was performed by using the primer extension KASP® genotyping assay.
The agreement with “Hardy-Weinberg equilibrium” for detected the distribution of CYP2C9 genotype
was proved by (X2 – test) .The CYP2C9*2 , CYP2C9*3 and CYP2C9*5 alleles variant were identified in
Iraqi population , whereas the CYP2C9*4 was absent , The results revealed that there was non- significant
association observed in all CYP2C9 genotypes among Iraqi ethnic groups .then The CYP2C9 genotypes
results in our study were compared to other populations and The results exhibited that the frequency of Iraqi
population CYP2C9 variants were comparable with other countries and ethnic groups. This is the first report
of CYP2C9 variants from Iraq; the results are assist as a database on CYP2C9 gene polymorphisms and
clinical data baseline for dosing and avoiding adverse reactions of drugs metabolized by CYP2C9 in Iraqis
patients.