A novel Screening Methods using SSCP –PCR to Detect Variations Among three Varieties of Trigonella Foenum Graecum L. Collected from Different Regions in Iraq

Abstract

Fenugreek is one of the most commonly used herbal medicinal plants. Another benefit of fenugreek seeds
is that it is used to enhance the flavor, color and texture of fruit. Fenugrek comprises a number of chemical
components such as trigonellines, choline, flavonoids and fixed oils. SSCP –PCR is useful to reach rare and
endemic species’ genetic diversity and also to resolve genetic links between populations. . PCR amplification
of DNA. Genotyping of ITS4 was performed using a polymerase chain reaction technique, followed by singlestrand
conformation polymorphism. Accordingly, these DNA polymorphisms were confirmed using DNA
sequencing. The results appeared that the presence of three different haplotype patterns named according
to the number of bands were 4-bands, 3-bands, 2 –bands using SSCP –PCR technique. The pattern of all
resolved SSCP bands can however be difficult to establish using gel visualisation alone. This polymorphisms
of DNA must then be verified by the use of DNA sequencing. The findings of the sequence shown that the
haplotypes of the ITS region have been identified by several SNPs.
Conclusion: our findings indicated that SSCP-PCR technique is more informative for evaluation of genetic
diversity and relationships among fenugreek populations.