Thirteen New Yeast Strains Isolated from Cancer Patients in Basrah-Iraq by ITS rDNA Sequencing

Abstract

Yeast isolates were grown on CHR OMagar Candida medium to detect different types of the yeast species, and then the unidentified isolates were diagnosed by using molecular analysis of ITS region. From a total of 54 yeast isolates, 37 isolates appeared as different species of the genus Candida, the rest of species belong to Hanseniaspora, Kazachstania, Kluyveromyces, Magnusiomyces, Pichia and Saccharomyces. 13yeasts species in this study reported as new strains in Gen Bank, also species belong to C. pronicula, H. uvarum, K. exigua, K. marxianus, M. capitatus, Magnusiomyces sp., P. kudriavzevii, P. manshurica and S. bayanus x S. cerevisiae isolated for the first time in Iraq. We concluded that there are new emergent species of the yeasts inhabit the oral cavity of cancer patients undergoing chemotherapy, and the results showed that the molecular method provide a good approach for the identification.