Neuroprotective Effect of Moringa Oleifera Extract on Acetamiprid Induced Neurotoxicity and Apoptosis in Albino Rats

Abstract

Acetamiprid (ACMP) can cause neurotoxicity and induces neuronal apoptosis. The ameliorative effects of
Moringa Oleifera (MO) seed extract against the neurotoxicity induced by ACMP in adult male albino rats
were investigated. Forty male albino rats were divided into 4 equal groups. The first group served as a control,
the second group administered ACMP 26.8mg/kg b.w. (representing 1/10 LD50) by gavage. The third group
was given combination of ACMP 26.8 mg/kg b.w. and MO seed extract 150 mg/kg b.w. the fourth group was
given MO extract only 150 mg/kg b.w. for 28 consecutive days. Results proved the efficacy of MO extract
as a neuroprotective agent through the reduction of the oxidant parameter malondialdhyde (MDA) content
by 26.9%, significant increase of reduced glutathione content (GSH) by 21.4%, and elevation of catalase
(CAT) and superoxide dismutase (SOD) activities by (62.2% and 40.8%) respectively, compared to ACMP
intoxicated group. Consequently, gene expression analysis for Bax and NBN genes was downregulated
by 54.5% and 36.3% respectively in MO treated rats (group III) compared with ACMP intoxicated group.
Moreover, restoration of the pathological tissue injuries was noticed. In conclusion, MO proved to be an
effective neuroprotective and anti-apoptotic agent against ACMP-induced toxicity.