Phylogenetic Analysis Related to Hepatitis B Virus based on Region Surfaces in Tuban, East Java Province, Indonesia

Abstract

Hepatitis B virus (HBV) is a virus cause liver disease. This virus could be transmitted through blood product,
and it becomes the major problems in developing countries. Genotype distribution of HBV depends on
geographical characteristics. Here, we reported HBV genotyping analysis of blood donors with positive
Hepatitis Surface Antigen (HbsAg) at Indonesian Red Cross Foundation in Tuban, East Java. Total 150
sera were collected from blood donors at Indonesian Red Cross Foundation in Tuban, East Java. Enzyme-
Linked Immunosorbent Assay (ELISA) was conducted by PRISM®HBsAg kit (ABBOTT) according to
the manufacture’s instruction, to determine sample that has positive HbsAg. The genotyping analysis was
done using polymerase chain reaction and sequencing method from 12 samples. Genetyx ver.10 software
was used for identification of HBV type. Analysis of HBV genotype revealed three different types of HBV.
HBV type B was the major HBV (50%). Interestingly, there was one unidentified HBV type suggested as a
novel subtype of HBV. Further genotyping analysis is needed to confirm novelty of the unidentified HBV
type. Analysis of HBV genotype revealed three different types of HBV. HBV type B was the major HBV
(50%). Interestingly, there was one unidentified HBV type suggested as a novel subtype of HBV. Further
genotyping analysis is needed to confirm novelty of the unidentified HBV type. This study was the first
evidence of blood donor’s genotyping analysis in Tuban.