The Effect of Adiponectin Recombinant in Rattus Norvegicus with Polycystic Ovarium Syndrome Model on Anti-Müellerian Hormone Expression

Abstract

Background: Polycystic Ovary Syndrome (PCOS) is an important disease that causes various health
problems in women. Ovulation - infertility disorders are found in 60-85% woman with PCOS. Anti-
Muellerian Hormone (AMH), a protein from Transforming Growth-b Family, often increased in patient
with PCOS and associated with severity and poor prognosis for assisted reproductive outcome. Dietary
and lifestyle changes are the first-line therapies but consistent application of this method is difficult to
attain for people with PCOS. Adiponectin, a product by adipose cells have a significant reduction in
obese and women with PCOS. The purpose of this study was to see the differences in AMH expression
between mice with polycystic ovary model treated with adiponectin recombinant and mice with
polycystic ovary model treated with placebo and control.
Method: This research is an experimental study with a Post Test Only Control Group design. The
sample of this study was 3 months female Rattus norvegicus Wistar strain weighing 110-120 g. The
sample size was 36, which were divided into three groups, K0 (control group of mice with polycystic
ovary model), K1 (group of mice with polycystic ovary model that received placebo injection), and K2
(group of mice with polycystic ovary model that received 5 μg/ml injection of recombinant adiponectin).
Polycystic ovary model made by injecting 10 mg/Kg body weight testosterone propionate for 14 days.
The injection is carried out at the proestrous stage. The three groups of mice in a period of 3 weeks after
treatment, were surgically removed according to the sampling procedure, then the ovaries were given a
code and immunohistochemical staining was performed to see the AMH expression in the ovaries. The
data were collected, then statistical analysis was carried out using SPSS software.