Molecular Study Using Real Time PCR to Detect Influenza Viruses (Flu A, Flu B and RSV) in Patients at Ramadi Hospital

Abstract

Background: Because of the increasing incidence of infections with different types of influenza of
various types, type A, B, and SRV, this study included investigating the type of infection in influenza
in women and men in different age groups and making a comparison between them. Methodology: 90
blood samples were collected, including 30 samples for men 30 samples for women showing clinical
symptoms of infection, and 30 samples from people with clinical symptoms under control. These
samples were placed in tubes containing an anticoagulant to ensure that blood does not clot DNA/RNA
extraction kit, CerTest, Spanish Company and detection by Flu A, Flu B and RSV, CerTest, Spanish
Company by using real time PCR. Result: Table 1 shows the investigation of some types of influenza
in women who showed symptoms of infection and who are between 20 to 40 years old, where the
frequency of infection in women between the ages of 20-30 years was high with types Flu A, SRV
compared with control and did not show infection with type B for the same Age group . As for women
aged between 30-40 years, the frequency of type B infection was high compared to control, and there
were no infections with Flu a and SRV for the same age group .