Comparison of the Efficiency of GlobalfilerTM IQC PCR Amplification Kit and Powerplex® Fusion 6C System with Half-Volume Reactions for Forensic Trace DNA Testing
DOI:
https://doi.org/10.37506/ijfmt.v16i3.18246Keywords:
Forensic genetic; DNA typing; short tandem repeats; human identification; GlobalFilerTM IQC PCR Amplification Kit; PowerPlex® Fusion 6C System.Abstract
Two major challenges in daily and routinely forensic genetics test are samples with low or very low quantities
of DNA and high cost of processing. To overcome these obstacles, a reduction in reaction volume to half would
offer a substantial benefit, but even so it should provide complete genetic profiles without conceding the quality
of the results. The aim of this study is to test the robustness of two commercially available kits, GlobalFilerTM
IQC PCR Amplification Kit (GF-IQC) and PowerPlex® Fusion 6C System (P-6C), with half volume reaction in
accordance with SGWDAM guidelines. GF-IQC showed minimum and stochastic threshold of 45 and 610 RFU,
while P-6C demonstrated 60 and 670 RFU of those values respectively. The P-6C can tolerate to many inhibitors
including melanin, hematin, and humic acid. Both kits showed the same performance on case work profiling and
the sensitivity of allele detection of P-6C was not significantly different from that of GF-IQC (p=0.144). The results
of this study demonstrated that GF-IQC and P-6C with half volume reaction can produce good quality profiles and
could be applied for forensic DNA examination.
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