Green Synthesis of Copper Nanoparticles using Green Tea and Neem Formulation and Assessment if its Antimicrobial Effects
DOI:
https://doi.org/10.37506/ijfmt.v16i4.18550Keywords:
Copper, nanoparticles, neem, green tea, plant extract, antimicrobial activityAbstract
Aims: Nanotechnology is the science which is about manipulating matter, atom by atom and is associated with
particles smaller than 100 nm in size. Copper nanoparticles are used mainly due to its surplus amount, low cost,
easy availability and biocompatible property. Green synthesis of copper nanoparticles is very simple, economical
and eco-friendly method that does not involve any toxic chemicals. The aim of our study is green synthesis of
copper nanoparticles using green tea and neem formulation and assessment of its antimicrobial effects.
Place and Duration of Study: Sample: Department of Nanotechnology, Saveetha dental college and hospitals,
between December 2020 and January 2021
Methodology: 20 mM of copper sulphate solution is mixed with 40mL of plant extract and 60 mL of distilled
water was added and made it into 100 ml solution. Once the copper nanoparticles are synthesized the solution
is characterized using UV- vis-spectroscopy and was scanned in double beam UV-vis- spectrophotometer from
300 nm to 700nm wavelength. The antimicrobial property of copper nanoparticle is evaluated by agar well
diffusion method.
Results: The colour change from green to brown and peak observed in UV-vis- spectrophotometer was associated
with the synthesis of copper nanoparticles. Copper nanoparticle from green tea and tea extract has good
antimicrobial activity against S.mutans, C.albicans, E.faecalis, S, aureus.
Conclusion: Copper nanoparticles can be efficiently synthesised from green and neem formulation.hese copper
nanoparticles showed good antibacterial properties and are effective against oral pathogens
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https://creativecommons.org/licenses/by-nc/2.0/deed.en