Green Synthesis of Copper Nanoparticles using Green Tea and Neem Formulation and Assessment if its Antimicrobial Effects

Authors

  • Anjali Anna Thomas
  • Remmiya Mary Varghese
  • S Rajeshkumar

DOI:

https://doi.org/10.37506/ijfmt.v16i4.18550

Keywords:

Copper, nanoparticles, neem, green tea, plant extract, antimicrobial activity

Abstract

Aims: Nanotechnology is the science which is about manipulating matter, atom by atom and is associated with
particles smaller than 100 nm in size. Copper nanoparticles are used mainly due to its surplus amount, low cost,
easy availability and biocompatible property. Green synthesis of copper nanoparticles is very simple, economical
and eco-friendly method that does not involve any toxic chemicals. The aim of our study is green synthesis of
copper nanoparticles using green tea and neem formulation and assessment of its antimicrobial effects.
Place and Duration of Study: Sample: Department of Nanotechnology, Saveetha dental college and hospitals,
between December 2020 and January 2021
Methodology: 20 mM of copper sulphate solution is mixed with 40mL of plant extract and 60 mL of distilled
water was added and made it into 100 ml solution. Once the copper nanoparticles are synthesized the solution
is characterized using UV- vis-spectroscopy and was scanned in double beam UV-vis- spectrophotometer from
300 nm to 700nm wavelength. The antimicrobial property of copper nanoparticle is evaluated by agar well
diffusion method.
Results: The colour change from green to brown and peak observed in UV-vis- spectrophotometer was associated
with the synthesis of copper nanoparticles. Copper nanoparticle from green tea and tea extract has good
antimicrobial activity against S.mutans, C.albicans, E.faecalis, S, aureus.
Conclusion: Copper nanoparticles can be efficiently synthesised from green and neem formulation.hese copper
nanoparticles showed good antibacterial properties and are effective against oral pathogens

Author Biographies

  • Anjali Anna Thomas

    Post Graduate, Department of Orthodontics and Dentofacial Orthopaedics, Saveetha Dental College and Hospital, 162, Poonamallee high road,Chennai – 600077

  • Remmiya Mary Varghese

    Senior Lecturer, Department of Orthodontics and dentofacial Orthopaedics,
    Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences,
    Chennai – 600077,

  • S Rajeshkumar

    Assistant Professor, Nanobiomedicine Lab, Department of Pharmacology,
    Saveetha Dental college and Hospital, Saveetha Institute of Medical and Technical Sciences,
    Chennai – 600077, Tamil Nadu, India.

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Published

2022-10-10

How to Cite

Green Synthesis of Copper Nanoparticles using Green Tea and Neem Formulation and Assessment if its Antimicrobial Effects. (2022). Indian Journal of Forensic Medicine & Toxicology, 16(4), 119-124. https://doi.org/10.37506/ijfmt.v16i4.18550