Zygosity Test of Twin Pairs Using 26 STR Loci in the Indonesian Population

Authors

  • Lobredia Zarasade
  • David S Perdanakusuma
  • Harianto Notopuro
  • Vira Saamia
  • Abdul Hadi Furqoni

DOI:

https://doi.org/10.37506/ijfmt.v16i4.18604

Keywords:

Monozygote; twins; STR; Loci

Abstract

Background: Twin research can play an important role in understanding the interplay between genes and
environment. Classifying twins as monozygotic (identical) or dizygotic (fraternal) is an essential first step in
conducting research that will yield valid and replicable findings. Identical twins (theoretically) share 100% of
their genes, while fraternal twins share, on average, 50% of their genes.1 This study aims to determine zygosity in
12 twins with Non-Syndromic Cleft Lip and Palate (NSCLP) discordant, concordant, and non-NSCLP.
Material and Methods: Blood samples from 12 twin pairs, which include of 8 twins Non-syndromic Cleft Lip
and Palate (NSCLP) discordant, 2 twins NSCLP concordant, and 2 twins non-cleft (normal). DNA extraction was
using Promega Kit and quantified was using Nano drops. DNA amplification was by PCR with 26 loci, which was
packaged in Powerplex Fusion 6C Kit. PCR products were sequenced using ABI3500 and the visualization can be
seen using Genemapper TM ID-X 1.6 software.
Results: The results show that each pair of 11 twins have a similar alleles in 26 STR loci, which means they are
monozygotic twins. Only 1 twin has a different alleles in 10 STR loci, which means they are dizygote.
Conclusion: Zygosity tests on MZ twins using STR loci has high similarity and sensitivity. This test would be more
accurate by analyzing a higher number of STR loci.

Author Biographies

  • Lobredia Zarasade

    Student at Post Graduate Doctoral Degree Program, Faculty of Medicine, Airlangga University,
    Indonesia.

  • David S Perdanakusuma

    Lecturer, Plastic Surgeon, Department of Plastic Reconstructive and Aesthetic Surgery,
    Faculty of Medicine, Airlangga University, Indonesia

  • Harianto Notopuro

    Lecturer, Department of Biochemistry,Faculty of Medicine, Airlangga University, Indonesia,

  • Vira Saamia

    Researcher, Center of Forensic Laboratory, Indonesian National Police, Indonesia

  • Abdul Hadi Furqoni

    Student at Post Graduate Doctoral Degree Program, Faculty of Medicine, Airlangga University and Researcher, Human Genetic and Forensic Laboratory, Institute of Tropical Disease, Airlangga University, Indonesia

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Published

2022-10-10

How to Cite

Zygosity Test of Twin Pairs Using 26 STR Loci in the Indonesian Population. (2022). Indian Journal of Forensic Medicine & Toxicology, 16(4), 303-307. https://doi.org/10.37506/ijfmt.v16i4.18604