The Role of Soil as Trace Evidence in Forensic Science: Methodologies and Applications

Authors

  • Tewodros Assefa National Forensic Science University , Gandhinagar, Gujarat
  • Surbhi Mathur School of Forensic Science, National Forensic Sciences University

DOI:

https://doi.org/10.37506/arsd9w59

Keywords:

forensic soil analysis, trace evidence, physical methods, chemical methods, interdisciplnary collabration

Abstract

In forensic science, soil as trace evidence can play a vital role of establishing connection between the suspect, victims, any object with a particular area based on the characteristics of soil. Forensic soil investigation is the analysis of soil evidence in the service of crime solving, environmental monitoring, and geologic exploration. Analysts use a combination of geological, ecological, biological, or chemical methods to identify soil origin, link evidence to crime scenes, or reconstruct events. This study aims to conduct a comprehensive review of existing literature on soil as trace evidence, with a focus on its forensic applications. This covers various aspects of forensic soil investigation, including Physical methods (grain size analysis, density gradient measurement), chemical processes (organic matter analysis; loss on ignition (LOI), pH), biological methods (DNA analysis, microbial community analysis), and mineralogical analysis (SEM-EDX, XRD), For soil analysis, these methods are evaluated for their applicability and limitations. It concludes by arguing the importance of assembling multiple disciplines for effective forensic soil investigation. It provides a helpful resource for forensic scientists and researchers, as well as for investigators and practitioners in forensic science.

 

Author Biographies

  • Tewodros Assefa, National Forensic Science University , Gandhinagar, Gujarat

    PhD. Scholar, Department of Forensic Physics, School of Forensic Science, National Forensic Sciences University, Gandhinagar, Gujarat, India.

  • Surbhi Mathur, School of Forensic Science, National Forensic Sciences University

    Associate Professor, School of Forensic Science, National, Forensic Sciences University, Gandhinagar, Gujarat, India

References

Corrêa RS, Melo VF, Abreu GGF, Sousa MH, Chaker JA, Gomes JA. Soil forensics: How far can soil clay analysis distinguish between soil vestiges? Sci Justice. 2018 Mar 1;58(2):138–44.

Cubbage HR, Macey C, Scott KR. Macroscopic assessment of environmental trace evidence dynamics in forensic settings. Sci Justice [Internet]. 2023;63(3):376–86. Available from: https://doi.org/10.1016/j.scijus.2023.03.004

Raven MD, Fitzpatrick RW, Self PG. Trace evidence examination using laboratory and synchrotron X-Ray diffraction techniques. In: Geological Society Special Publication. Geological Society of London; 2021. p. 167–79.

Fitzpatrick R, Raven M, Self P. The role of pedology and mineralogy in providing evidence for 5 crime investigations involving a wide range of earth materials. Episodes. 2017;40(2):148–56.

Fitzpatrick RW, Donnelly LJ. An introduction to forensic soil science and forensic geology: A synthesis. In: Geological Society Special Publication. Geological Society of London; 2021. p. 1–32.

Sharma V, Acharya R, Sarkar A, Bagla HK, Pujari PK. Chemical characterization of automobile windshield glass samples by nuclear and radio-analytical techniques namely SEM-EDX, ED-XRF, PIXE, PIGE, and INAA and potential of external (in air) PIGE and INAA in conjunction with chemometrics for glass forensics. J Anal At Spectrom. 2023;

Tambuzzi S, Gentile G, Primavera R, Muccino E, Zoja R. Pilot Application of SEM/EDX Analysis on Suspected Cigarette Burns in a Forensic Autopsy Case of Child Abuse. Am J Forensic Med Pathol. 2024 Jun 1;45(2):135–43.

Rus Din RD, ISHAR SM, NAGANATHAN H. Physical and Chemical Characteristics of Oil Palm Plantation Soil: A New Lead in Forensic Investigation. J Sains Kesihat Malaysia. 2021;19(01).

Croft DJ, Pye K. Multi-technique comparison of source and primary transfer soil samples: An experimental investigation. Sci Justice - J Forensic Sci Soc. 2004;44(1):21–8.

Ruffell A. Forensic pedology, forensic geology, forensic geoscience, geoforensics and soil forensics. Forensic Sci Int [Internet]. 2010;202(1–3):9–12. Available from: http://dx.doi.org/10.1016/j.forsciint.2010.03.044

Morgan RM, Bull PA. Forensic geoscience and crime detection Identification, interpretation and presentation in forensic geoscience. Vol. 127, MINERVA MED LEG. 2007.

Youwai S, Makam P. Computer Vision for Particle Size Analysis of Coarse-Grained Soils. In 2023.

Itamiya H, Sugita R, Sugai T. Investigation on forensic soil analysis using quartz shapes and surface microtextures. J Geol Soc Japan. 2020 Aug 15;126(8):411–23.

Chaperlin K, Howarth PS. Soil comparison by the density gradient method - A review and evaluation. Forensic Sci Int. 1983;23(2–3):161–77.

Dudley RJ. The Use of Density Gradient Columns in the Forensic Comparison of Soils. Med Sci Law. 1979;19(1):39–48.

Nataraja Moorthy T, Wan Mohamad Fuad WNS. Study on Subsoil Profile by Density Gradient Tube Technique at Royal Malaysian Police College Campus in Forensic Perspective for Crime Investigation. Coll SADHANA-Journal Bloom Res [Internet]. 2012;5(1):501. Available from: https://s3.amazonaws.com/academia.edu.documents/30547185/Syazwani_-_College_Sadhana_-_soil_-_2012.pdf?AWSAccessKeyId=AKIAIWOWYYGZ2Y53UL3A&Expires=1544982326&Signature=KY05zqXp17kfEdH5gluLAAx5aAc%3D&response-content-disposition=inline%3B filename%3DStudy

Osterburg JW. Forensic science: An introduction to criminalistics. J Crim Justice. 1985 Jan;13(1):88–90.

Petraco N, Kubic T. A density gradient technique for use in forensic soil analysis. J Forensic Sci. 2000 Jul;45(4):872–3.

Fitzpatrick RW. Soil: Forensic Analysis. Wiley Encycl Forensic Sci. 2009;(September 2009).

Cortés-Esquivel JL, Herrera-Silveira J, Quintana-Owen P. Organic Matter Content in Mangrove Soils from a Karstic Environment: Comparison between Thermogravimetric and Loss-on-Ignition Analytical Techniques. Forests. 2023;14(7).

Bensharada M, Telford R, Stern B, Gaffney V. Loss on ignition vs. thermogravimetric analysis: a comparative study to determine organic matter and carbonate content in sediments. J Paleolimnol [Internet]. 2022;67(2):191–7. Available from: https://doi.org/10.1007/s10933-021-00209-6

Claassen B, Redman E, Schwartz T. Determining the Minimum Weight of Soil Samples for Forensic Geological Analysis. 2022;12(1):22–46.

Hoogsteen MJJ, Lantinga EA, Bakker EJ, Groot JCJ, Tittonell PA. Estimating soil organic carbon through loss on ignition: Effects of ignition conditions and structural water loss. Eur J Soil Sci. 2015;66(2):320–8.

Dudley RJ. A Simple Method for Determining the pH of Small Soil Samples and its use in Forensic Science. 1975;(1 976).

Nel T, Clarke CE, Hardie AG. Comparison of Soil pH and Exchangeable Cation Quantification by Various Wet Methods with Near- and Mid-Infrared Spectroscopy Prediction. Commun Soil Sci Plant Anal. 2023;54(17):2425–38.

Bonetti J, Quarino L. Comparative forensic soil analysis of new jersey state parks using a combination of simple techniques with multivariate statistics. J Forensic Sci. 2014;59(3):627–36.

Dawson LA, Macdonald LM, Ritz K. Plant wax compounds and soil microbial DNA profiles to ascertain urban land use type. Geol Soc Spec Publ. 2021;492(1):249–60.

Young JM, Rawlence NJ, Weyrich LS, Cooper A. Limitations and recommendations for successful DNA extraction from forensic soil samples: A review. Sci Justice [Internet]. 2014;54(3):238–44. Available from: http://dx.doi.org/10.1016/j.scijus.2014.02.006

Giampaoli S, Berti A, Di Maggio RM, Pilli E, Valentini A, Valeriani F, et al. The environmental biological signature: NGS profiling for forensic comparison of soils. Forensic Sci Int. 2014;240.

Demanèche S, Schauser L, Dawson L, Franqueville L, Simonet P. Microbial soil community analyses for forensic science: Application to a blind test. Forensic Sci Int [Internet]. 2017;270:153–8. Available from: http://dx.doi.org/10.1016/j.forsciint.2016.12.004

Sangwan P, Nain T, Yadav P, Sharma N. Molecular Approaches in Soil Microbial Analysis: Forensic Perspective. Biosci Biotechnol Res Asia. 2023;20(2).

Jurkevitch E, Pasternak Z. A walk on the dirt: Soil microbial forensics from ecological theory to the crime lab. FEMS Microbiol Rev. 2021;45(2):1–18.

Moreno LI, Mills DEK, Entry J, Sautter RT, Mathee K. Microbial metagenome profiling using amplicon length heterogeneity- polymerase chain reaction proves more effective than elemental analysis in discriminating soil specimens. J Forensic Sci. 2006;51(6):1315–22.

Concheri G, Bertoldi D, Polone E, Otto S, Larcher R, Squartini A. Chemical elemental distribution and soil DNA fingerprints provide the critical evidence in murder case investigation. PLoS One. 2011;6(6):4–8.

Pye K. Forensic examination of rocks, sediments, soils and dusts using scanning electron microscopy and X-ray chemical microanalysis. Geol Soc Spec Publ. 2004;232(1942):103–22.

Fitzpatrick RW, Raven MD. How Pedology and Mineralogy Helped Solve a Double Murder Case: Using Forensics to Inspire Future Generations of Soil Scientists. Soil Horizons. 2012;53(5):14.

Kikkawa HS, Naganuma K, Kumisaka K, Sugita R. Semi-automated scanning electron microscopy energy dispersive X-ray spectrometry forensic analysis of soil samples. Forensic Sci Int [Internet]. 2019;305(September):109947. Available from: https://doi.org/10.1016/j.forsciint.2019.109947

Pirrie D, Crean DE, Pidduck AJ, Nicholls TM, Awbery RP, Shail RK. Automated mineralogical profiling of soils as an indicator of local bedrock lithology: A tool for predictive forensic geolocation. In: Geological Society Special Publication. 2021.

Philip S, Singh N. Comparative Soil Analysis by Scanning Electron Microscope: A Forensic Perspective. Int J Emerg Technol [Internet]. 2020;11(2):915–23. Available from: www.researchtrend.net

Cengiz S, Karaca AC, Çakir I, Üner HB, Sevindik A. SEM-EDS analysis and discrimination of forensic soil. Forensic Sci Int. 2004;141(1):33–7.

Pitts KM, Clarke RM. The forensic discrimination of quartz sands from the Swan Coastal Plain, Western Australia. Forensic Sci Int Reports [Internet]. 2020;2(August):100130. Available from: https://doi.org/10.1016/j.fsir.2020.100130

Neha Yadav, Lav Kesharwani. Analytical Instrument and its Utilization in Soil Forensic: A Review. Indian J Forensic Med Toxicol. 2022;17(1):48–52.

Newland TG, Pitts K, Lewis SW. Multimodal spectroscopy with chemometrics for the forensic analysis of Western Australian sandy soils. Forensic Chem. 2022;28:1–26.

Hachem M, Sharma BK, El Naggar A, Pilankar I, Anwar N. Systematic approaches for soil analysis in forensic investigation. 2020 Adv Sci Eng Technol Int Conf ASET 2020. 2020;

Ruffell A, McKinley J. Geoforensics [Internet]. Wiley; 2008. Available from: https://books.google.co.in/books?id=f3UCEAAAQBAJ

Fitzpatrick RW, Raven MD, Forrester ST. A systematic approach to soil forensics: Criminal case studies involving transference from crime scene to forensic evidence. Crim Environ Soil Forensics. 2009;(2):105–27.

Gamboa-Razo DC, Tierra-Auquilla JL. Caracterización de suelos en relación al carbono orgánico total almacenado, en las microcuencas del río Quinuaquira y quebrada Curiquín parroquia el Altar del cantón Penipe provincia de Chimborazo. [Internet]. Riobamba: Universidad Nacional de Chimborazo, 2016.; 2016. Available from: http://dspace.unach.edu.ec/handle/51000/3112

Schwartz TR, Rothenberg DS, Clark BL. Trace evidence recognition, collection, and preservation. Handb Trace Evid Anal. 2020;1–31.

Pirrie D, Dawson L, Graham G. Predictive geolocation: Forensic soil analysis for provenance determination. Episodes. 2017;40(2):141–7.

Young JM, Austin JJ, Weyrich LS. Soil DNA metabarcoding and high-throughput sequencing as a forensic tool: Considerations, potential limitations and recommendations. FEMS Microbiol Ecol. 2017;93(2).

Downloads

Published

2024-12-03

How to Cite

The Role of Soil as Trace Evidence in Forensic Science: Methodologies and Applications. (2024). Indian Journal of Forensic Medicine & Toxicology, 19(1), 38-44. https://doi.org/10.37506/arsd9w59